You do not need an R4 card or a twilight menu wrapper to boot the game; it runs directly off your 3DS SD card.
The game relies on a four-button control scheme. On the original DS hardware, A and B controlled adult Mario and Luigi, while X and Y controlled the babies. Players manage two pairs of characters simultaneously, splitting them up to solve intricate environmental puzzles or combining them to unleash devastating "Bros. Items" in turn-based combat. Critical Reception and Impact
You place the .nds ROM on your SD card and use these tools to generate a "fake" CIA that acts as a launcher. 2. TWiLight Menu++